ABSTRACT
Objective To investigate the protective effect of Shen Fu Injection (SFI) on cardiac function in sepsis rats and to explore the possible mechanism.Methods Forty SD male rats were randomly divided into 4 groups,namely normal control group,sham operation group,model group,SFI group.The sepsis model was established by cecal ligation and puncture (CLP).Thirty-six hours later,the arterial blood and left ventricular myocardium tissues were collected,and then the serum levels of tumor necrosis factor(TNF)-α and interleukin(IL)-1 were detected and the levels ofphosphorylated p38-mitogen-activated protein kinase (p-p38MAPK) and p38-mitogenactivated protein kinase (p38MAPK) in the supernatant of myocardial homogenate were detected.Results Thirty-six hours after modeling,left ventricular ejection fraction (LVEF) and left ventricular fractional shortening(LVFS) of the rats in the model group were significantly lower than those in the sham operation group (P ＜ 0.05).The heart function in SFI group was much improved compared with the model group (P ＜ 0.05).The serum TNF-α and IL-1 levels as well as p-p38/p38MAPK level in the supernatant of myocardial tissue of SFI group were lower than those in the model group (P ＜ 0.05).There were no significant differences of the above indexes between the sham operation group and the normal control group (P ＞ 0.05).Conclusion SFI has protective effect against sepsis myocardial injury.The mechanism may be related with the inhibition of p38MAPK phosphorylation in the myocardium,thereby reducing the release ofinflammatory cytokines in the pathway.
ABSTRACT
Objective To discuss the relationship of miR-21 expression and IL-12 level in patients with chronic obstructive pulmonary disease.Methods Chosed 80 patients with suspected chronic obstructive pulmonary disease(COPD)and 50 cases as control group.Real-time RT-PCR was performed to determine the expression 1 evels of miR-2 1 of peripheral blood mono-nuclear cells of three groups.To detect IL-1 2 level by ELISA method.Results There were no statistical difference among GLU,TC and TG level among stable phage,acute phage of COPD and control group(F=0.341~1.542,P>0.05).Howev-er miR-21 expression and IL-12 level had statistical difference (F=8.951~15.017,P<0.05).The expression 1evels of miR-2 1 of peripheral blood mononuclear cells of acute phage group were apparently higher than stable phage,health group(P<0.05).The concentration of IL-12 in serum of acute COPD group was higher than other two groups(P<0.05),compared with stable COPD group and health group.IL-12 level was positiveiy related to miR-21 expression (r=-0.381~0.496). Conclusion miR-2lmay be involved in the regulation of IL-12 expression.miR-21expression may be closely related to the pathogenesis of COPD.